Recently, the research team of Wu Liuji, a agricultural college of Henan Agricultural University in Agricultural University, made breakthroughs in the research of plants (corn) endogenous peptides. On November 11, 2024(IF: 23.7) Published an academic paper entitled "A Comprehensive Atlas of Endogenous Peptides in Maize".For the first time, the study constructed the panoramic diagram of the plant (corn) endogenous peptide, analyzing the dynamic expression mode and tissue specific distribution of endogenous peptides in different development stages, and revealing the presence of an internal analogenic peptide in the organism.mechanism.The study will laid an important foundation for understanding the regulation mechanism of plant -endogenous peptides and provides new perspectives for plant biology research. At the same time, it also provides excellent candidate resources for future use of endogenous peptides for crop improvement.
Internal peptide, usually consisting of 2 to 100 amino acids as an important regulatory factor for plant growth, development and coercion response, positiveWith more and more attention.These small molecules play an important role in various biological processes, including cell signal transmission, growth control and defense reactions.However, so far, the research on the source peptide in the plant is mainly concentrated in a single small peptide, and it usually only pays attention to its functions in specific biological processes.Through group learning, systematic research can be conducted. However, the current research of group studies is mainly concentrated in the research of genomes, transcription groups and protein groups.These studies provide a new field of vision for revealing the role of genes or proteins in various biological processes, and also reveal the complex regulatory network of plant development or adversity response.However, compared to the genome, transcription group, and protein group, the relatively lagging research of the polypeptide group has not yet been systematically studied for the entire growth and development period of the plant in the entire genome range of the plant.
This study uses the extraction and appraisal method of plant endogenous peptides established by the research group (Wanget Al., Molecular Plant, 2020).13 kinds of corn tissue (39 samples): including 6-17, 3 -leaf younger tablets, 8 -leaf mature leaves, 5 -day roots, 7 -day side roots, 19 -day nutritional conidiaFlower silk, female ears, leaflets, ebbing ecstasy for 8 days after pollination, and 20 days after pollination, a more comprehensive corn growth and development end source peptide graph is generated to identify 6,100 non -redundant endogenous peptides.It was found that the corn internal peptide showed different expression patterns in different development stages.According to its expression pattern, endologically, the endogenous peptide can be divided into tissue specificity, tissue enhancement and hybrid peptide.Among each organization, there are about 21 to 680 tissue -specific peptides, 2 to 877 tissue enhanced peptides and 19-1000 mixed peptides. The endogenous peptide shows a diverse expression mode during the nutrition and reproductive stage, showing the cornHigh complexity and dynamic characteristics of the polypeptide group.In addition, the expression of tissue enhancement and hybrid peptides in the nutrition and reproductive stage is more dynamic, while the expression of tissue specific peptides is relatively stable (Figure 1).
Figure 1. Corn endogenous peptide quantitative analysis, abundance mode, and chromosomal distribution conditions
Previous research showed that there was a weak positive correlation between the transcription group and the protein group.However, the correlation between the polypeptide and the protein group is not clear.This study found that the correlation between the Spearman of the endogenous peptide and the source of the source is between -0.06 to 0.21, and the correlation between the endogenous peptide and the adjacent protein is close to zero, indicating that the tissue-specific regulation of the internal peptide may be different from the protein that is different from the protein.The regulatory mechanism, and even the endogenous peptide may play the opposite function in the process of the same biological regulation.The significant low correlation between endogenous peptides and source protein, and the non -conservative expression of its related proteins may be attributed to multiple factors, such as transit peptide from the synthetic tissue to other partsAfter translation modification, and dynamic interaction between the in vivo -in vivo and the protein (Figure 2).
Figure 2. Full genome of corn inner source peptide and source protein expresses analysis
Henan Agricultural Universitys postdoctoral USman Ali and young teachers Tian Lei as the first author of the dissertation, Professor Wu Liuji is the author of the dissertation, young teachers Wang Shunxi, Luo Weiwei, doctoral graduate Zhang Jinghua, master of master Tang Ruihong and Liu Shanshan participated in thisItem research work.The study was strongly supported by the team leader, Professor Chen Yanhui, and received funding from the National Natural Science Foundation of China-joint key projects, national key research and development plan projects, and national natural science funds.
The head of the Wu Liuji research team has been engaged in the study direction of corn anti -inverse biology and new small peptides. In recent years, it has made innovative research on the excavation and functional research of new small peptidesWork.Related results are in the internationally renowned journal IMETA (2024), Cells subsidiary Cell Reports (2024), the TOP journal Molecular Plant (2022,2020), Trends In Plant Science (2023), and the authoritative journal of the authoritative journalPlant BioteChnology Journal (2019, 2021), Journal of Integrative Plant Biology (2022), Plant (2024) and other magazines published 35 SCI papers; hosting 6 National Natural Science Foundation of China, including the National Natural Science Foundation of China, including Gold Union Key Project 1 itemEssence
Thesis link:
http://doi.org/10.1002/imt2.247
