Introduction
The quantification of the number of ancestral cells in an organ, tissue or cell group is essential for understanding the development of multi -cell creatures and the balance of body balance. The previous research depends on the marking genes expressed in the ancestral cells.However, this strategy is often blocked due to lack of ideal tags.
On February 20, 2024, the Hexiong Lei team of Sun Yat -sen University in Nature Methods published online entitled " A Statistic Method for Quantifying Progenitor CellsReveals INCIPIENENENET CELL FATE Commitments "research papers, The study proposes a general statistical method to explore the cell system development tree that records the history of cell division and quantifies any tissue or cell group in the organism.The ancestral cells, even if the ancestral cell -specific labeling is lacking.
This method is called a targeted gathering analysis (TARCA). Calculating two random sampling cells in the tissue are in the tissue -specific single -system evolutionary branches and convergenceThe probability of knot.The countdown of this probability can be used to measure the number of ancestral cells in the tissue.Both mathematical models and computer simulation prove the high accuracy of Tarca, and then verify the real data of nematodes, fruit flies and mice, and all data have related cell system development trees. The study further shows that Tarca can be used to identify the genealogy -specific raising genes during the occurrence of embryos, revealing the commitment of mouse embryos in early cell fate.
Genetic diversity has long been the core concept of understanding biological population evolution. In multi -cell creatures, cell groups that perform specific functions usually come from a group of ancestral cells, and the number of ancestral cells determines the diversity of the cell group.Among the biological groups, the cloned expansion to a small non -non -non -non -non -non -non -non -non -non -non -non -non -asexual group is very common. The low -spectrum diversity of cell groups may increase the susceptibility of related tissues to mutations in harmful body cells. A prominent example is a human vascular system composed of 20,000 to 200,000 stem cells or multi -energy ancestral cells. This hematopoietic stem cells or multi -energy ancestral cell groups are important to prevent the increase in the increase in blood in the blood when they are young, and explains the causes of sudden reduction in the diversity of blood spectrum after 70 years of age.This age -dependent clone amplification is also observed in a variety of physical organs or organizations. Therefore, a good way to quantify the number of ancestors of ancestral cells is valuable to understand the stability of tissue.This is also useful for research on embryonic development, because embryo development involves complex cell groups amplification and differentiation.
The most advanced strategy to track the ancestral cells of cell ancestral cells depends on the ancestral cell -specific expression of the label. These marks can be designed as a marked offspring cell group. Although there are successful cases, this strategy is limited to dependence on the ideal mark, which has a specific expression among all the ancestral systems of the stove cell group.In addition, this method is confirmed, not exploratory, because it needs to understand the ancestor cells in advance, and the ancestral cells revealed may not effectively promote offspring cell groups.In recent years, single cell RNA sequencing (SSSSSA-SEQ) has been used to track the expression of expression in development. Although the trajectory of vacation time based on expression of similarity provides information in many aspects, due to the complexity of high -dimensional expression data, they cannot accurately identify the ancestral cells of the cell group.
Recently drawing the technical progress of the cell system development tree diagram of complex creatures (including fruit flies, zebrafish, mice and humans), so thatTo cope with the above -mentioned challenges based on system development solutions. This is because in principle, the individual development of multi -cell creatures can be fully described by a systematic development tree. The root represents a coexistence, the fork represents the division cell division.Existence terminal cells.
Tarca theory (Credit: Nature Methods)
A systematic development method based on system development is used to estimate the number of ancestral cells of cell groups. This method bypasses the traditional experimental method depends on the pre -watched bids to track the main restrictions of the ancestral cells.A special advantage of Tarca is its versatility. It can analyze any cell group defined by organs, tissues, cell types or researchers, without having to understand the information of the ancestor cells in advance.Another advantage is that it plays a strong role in the development of a small part of the cells in complex organisms.In addition, Tarca can generate stable estimates in the development of wrong reconstruction cell system.In contrast, the counting method of the single -system evolution is highly sensitive to errors in system development and reconstruction. With the increasing popularity of reconstruction cell system, TARCA will be able to quantify the diversity of all tissue of complex creatures, including humans, which will be a quantitative model for understanding the development of plasticity and tissue age dependencies between individualsBase.
https://www.nature.com/articleS/S41592-02189-7
Jun
Types | Explore Jun
Article Source | "Inature"
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