Writing 丨 Wang Cong
Edit 丨 Wang Duoyu
Types 丨 Water TextRecently, Nature reported on the headline of the official website that a virusist used the virus he trained in the laboratory to treat his special case.
In 2020, 49-year-old Beya Halaci (Beata Halassy) discovered her recurred again, and she could no longer face a new round of chemotherapy.
Beya I was first diagnosed with tri -negative breast cancer (TNBC) in 2016. This is the most.The invasion and the most fatal breast cancer sub -type, she then accepted surgical resection and auxiliary chemotherapy.In 2018, a local recurrence of cancer occurred in the surgical resection site and recurred the recurrence cooker through surgery. However, there was still a small piece of hemodiamal swelling after the surgical resection site, which needed regular monitoring.In 2020, the phase comparison magnetic resonance imaging shows that this hemophilia structure has developed into a with a diameter of 2 cm, and skin infiltration has occurred.
At this time, Beya has no good treatment options, as a virusist at the University of Croatia (University of Zagreb), she, sheAfter studying the literature, he decided to do it himself, using a therapy that has not been proven in breast cancer - Tumache Crimine Virus therapy The process and treatment results were written into a paper, published in Vaccines journal [1].
Pumaging virus (OV) is a promising solid tumor treatment method.After injection in the tumor, the tumache virus can selectively target cancer cells without affecting normal cells. By copying in cancer cells, it causes cancer cells to crack.In addition, the tumache virus can further stimulate the immune system, so it can be used in combination with other cancer immunotherapy, such as immunohistochemical point inhibitors.At present, a total of 4 soluble virus therapy has been approved to be listed, and several cancers such as melanoma, colorectal cancer, and glue tumors are treated through tumor injection.But so far, no tumache virus therapy has been approved to treat breast cancer at any stage.
Biata It is not a solid virus expert, but the professional knowledge of cultivating and purifying viruses in the laboratory gives her confidence in trying tumache virus therapy.She chose two different tumache viruses - measles virus (MEV) and blisters virus (VSV).Both viruses can be infected with the type of cells of her tumor sources and have been used in clinical trials for soluble virus therapy.
Beya Before cultivating these two viruses, these two viruses have good safety -measles strains are widely extensiveFor childrens measles vaccine, and blisters virus virus can only induce minor influenza -like symptoms in the worst case.
Beya With the help of colleagues, the two solo virus trained in the laboratory (only research level, not clinical, not clinical,Level) directly injected into the tumor and performed multiple injections in 2 months.Her tumor doctor kept her monitoring during her self -treatment. Once a problem occurred, it would stop the tumache virus therapy and turn to conventional chemotherapy.
As shown in the figure below, she injected 7 times in 3 weeks and injected 3 VSVs before surgical resection of the tumor.The MEV was injected again under the surgical sutures, as preventive treatment.
Pumage virus treatment plan and ending
It seems to be effective: only 2 months of treatment, the tumor volume has been significantly reduced from 2.47 cm 3 to 0.91 cm 3. The tumor has changed from a hard, fixed nodules accompanied by skin inflammation to soft, no skin inflammation.Move nodules, and the invasive edge of the tumor faded.At the beginning of the treatment, it was extremely difficult to inject the tumache virus. At the end of the treatment, the tumor had softened significantly, making the injection easier.In addition, there is good tolerance during the treatment and no serious side effects occur.
The analysis of the tumor resection of surgical resection shows that lymphocytes are thoroughly infiltrated in the tumor, which indicates that the tumache virus is as expected to inspire the immune system to attack virus and tumor cells, CD20 positive, positiveB cells increased from 10%to 70%, CD8 positive T cells increased from 30%to 60%, and the infiltration of macrophages (CD68 positive cells) also increased.In addition, after the treatment of tumache virus, the tumor has transformed from PD-L1 negative to PD-L1 positive.After surgical resection of the tumor, Beya received a year of Tuskomo monoclonal antibody treatment (a anti -HER2 monoclonal antibody, used for the treatment of metastatic breast cancer).
Imaging evidence of the effect of the therapeutic therapeutic therapeutic virus
After the first diagnosis in 2016,Twenty -two months later, Biata experienced the recurrence of the first three -negative breast cancer, and 21 months later. She experienced the second triple -negative breast cancer recurrence.After the treatment of tumache virus, she has continued to ease 45 months without recurrence.Although this is an isolated case, this encourages the considering the use of tumache virus therapy as a new auxiliary therapy.
Beata Halassy
Biata I feel that she has the responsibility to publish this discovery. She wrote her treatment process as a paper, but was successively rejected by more than a dozen journals.Cancer patients give up conventional treatment to try similar methods, because cancer patients are particularly easy to try unconfirmed treatment methods.
Under her insistence, this paper was finally published in the Vaccines journal. or recurren Breast Cancer(Study on unconventional cases of recurrent breast cancer neo -tumor tumache virus therapy).
Baya believes that the publication of this paper is unlikely to cause people to imitate, because the cultivation and treatment of self -tumor virus requires many professional knowledge.And skill.not9/958
https://www.nature.com/articleS/d41586-024-03647-0
